Key attributes
SPECIFICATIONS
10 Tests/Box
Cat.No.: MG-CA-R007
The FHV-Ag test is a fluorescence immunoassay designed for use with an Immunofluorescence Analyzer to quantitatively determine the concentration of herpes virus antigen (FHV-Ag) in feline nasopharyngeal or ocular swabs. This test aids in the diagnosis and monitoring of feline herpes virus infections.
For in vitro diagnostic use only. For professional use only.
The FHV-Ag test utilizes a quantitative double antibody sandwich fluorescence immunoassay technique.
The intensity of the fluorescent signal correlates with the amount of FHV-Ag captured, and the concentration is expressed in Tu/ml.
10 individual sealed pouches, each containing:
Test Device
Desiccant pouch
1 ID Chip
Instructions for Use
10 cotton swabs
10 tubes of FHV-Ag Sample Buffer
10 Pipette tips
Immunofluorescence Analyzer
Timer
Pipette
Centrifuge
Store the test kit at 4–30°C until the expiration date.
Perform the test at 18–28°C after opening the Test Device.
Once the pouch is opened, the test should be completed within 30 minutes.
The test can be performed with a nasopharyngeal or ocular swab.
Specimen Collection:
Nasopharyngeal Swab: Insert the swab into the nasal cavity and gently rotate to collect the sample.
Ocular Swab: Gently swab the conjunctiva of the eye.
Ideally, the test should be performed immediately after specimen collection. If the test cannot be performed within 3 hours, store the specimen at 2–8°C for up to 48 hours. For long-term storage, specimens should be kept below -20°C.
Preparation for Testing:
Bring all materials to room temperature before use.
Thaw and mix frozen specimens thoroughly prior to testing.
Do not freeze and thaw specimens multiple times.
Use only clear, non-hemolyzed specimens.
Refer to the Immunofluorescence Analyzer Operation Manual for complete instructions.
Place the Test Device on a clean, level surface.
Insert the ID Chip into the meter and click “Read ID Chip.” Ensure that the Test Device lot number matches the ID Chip number.
Place the swab with the sample into the FHV-Ag Sample Buffer. Stir thoroughly and centrifuge for 1 minute (3000–5000 rpm).
Pipette 100 μl of the mixed sample into the sample well (S) of the Test Device, avoiding bubble formation.
Test Modes:
Standard Test: Click “Standard Test,” insert the Test Device into the meter holder immediately after adding the sample, and click “Start Test.” Select sample type “Swab.” The meter will automatically countdown and display the result.
Quick Test: Click “Quick Test,” start the timer immediately after adding the sample mixture, and leave the Test Device at room temperature (18–28°C) for 10 minutes. Insert the Test Device into the meter holder and click “Start Test.” Select sample type “Swab.” The instrument will automatically scan and display the result.
Results are displayed on the main screen and can be printed automatically or manually by clicking “Print.”
Each FHV-Ag Rapid Quantitative Test includes an internal control to ensure routine quality control. This internal control is performed with each patient sample. If the internal control fails, the meter will display an error message, indicating the need for a repeat test.
Reference Range for FHV-Ag in Feline Swabs:
Detection Range: 4.0–640 Tu/ml
| Result (Tu/ml) | Interpretation |
|---|---|
| ≤ 8 | Negative (-) |
| 8–15 | Suspected (±) |
| 15–40 | Weak Positive (+) |
| 40–200 | Medium Positive (++) |
| > 200 | Strong Positive (+++) |
Each laboratory should establish a reference range representative of the population being evaluated.
This kit is for in vitro diagnostic use only.
Inspect packaging and labels before use. Do not use if the pouch is damaged or if the vial appears compromised.
Do not use the Test Device beyond the expiration date.
Use a new pipette tip for each specimen to avoid contamination.
Be aware that technical or procedural errors, as well as substances in specimens not listed, may interfere with the test and result in erroneous outcomes.
