Key attributes
Test Quantity: 10 Tests/Box
Catalog Number: MG-CA-R023
The fNT-proBNP test is a fluorescence immunoassay designed for the quantitative measurement of feline N-terminal pro-brain natriuretic peptide levels in feline serum or plasma samples. It is intended to aid in assessing the risk of cardiac failure.
Note: For in vitro diagnostic use only. For professional use only.
This test utilizes a quantitative double antibody sandwich fluorescence immunoassay method. The intensity of the fluorescent signal correlates with the amount of fNT-proBNP captured, and the concentration is expressed in pmol/L.
Materials Provided
Each box contains:
10 individual sealed pouches, each containing:
A Test Device
A desiccant pouch
One ID Chip
Instructions for Use
10 cotton swabs
10 tubes of fNT-proBNP Sample Buffer
10 Pipette tips
Materials Required But Not Provided
Immunofluorescence Analyzer
Timer
Pipette
Centrifuge
Store the test kit at 4～30℃ until the expiration date.
Operate the test between 18 ~ 28℃ after opening the Test Device.
Perform the test within 30 minutes after the pouch is opened.
The test can be conducted using serum or plasma (EDTA anticoagulant is recommended).
Separate serum or plasma from blood within 3 hours of collection.
If the specimen appears severely hemolyzed, collect a new sample.
Perform the test immediately after specimen collection. If not possible, store the specimen at 2～8℃ for up to 72 hours. For long-term storage, keep specimens below -20℃.
Bring all materials to room temperature before testing. Thaw frozen specimens completely and mix well. Avoid repeated freezing and thawing. Use only clear, non-hemolyzed specimens.
Refer to the Immunofluorescence Analyzer Operation Manual for detailed instructions.
Place the Test Device on a clean, level surface.
Insert the ID Chip into the meter and select "Read ID chip." Ensure the Test Device lot number matches the ID Chip number.
Pipette 50 μl of prepared sample into the fNT-proBNP Sample Buffer, and mix gently to avoid vigorous agitation and foaming.
Pipette 100 μl of the mixed sample into the sample well of the Test Device, avoiding bubbles.
Test Mode Options:
Standard Test: Click "Standard Test," insert the Test Device into the device holder immediately, and click "Start Test." The meter will automatically countdown and display the test result.
Quick Test: Click "Quick Test," start the timer immediately after adding the sample mixture, and leave the Test Device at room temperature (18 ~ 28℃) for 15 minutes. Then, insert the Test Device into the meter holder and click "Start Test." The instrument will automatically scan the device and display the result.
Results are displayed on the main screen and can be printed automatically or manually by selecting "Print."
Each fNT-proBNP Rapid Quantitative Test includes an internal control for routine quality assurance. This internal control is checked every time a patient sample is tested. If the internal control returns an invalid result, an error message will display, indicating the need for a retest.
Detection range: 25 ~ 1600 pmol/L
Conversion factor: 1 pmol/L = 8.457 pg/ml
Reference Range:
≤ 50 pmol/L: Normal
50 – 100 pmol/L: Suspected
> 100 pmol/L: Abnormal
Note: Each laboratory should establish a reference range based on the specific population being evaluated.
For in vitro diagnostic use only.
Inspect packaging and labels before use. Do not use if the pouch is damaged, not well sealed, or if the vial is damaged or leaking.
Do not use the test device beyond the expiration date.
Use a separate pipette tip for each specimen.
Be aware that technical or procedural errors, as well as unlisted substances in blood specimens, may interfere with the test and lead to erroneous results.
